Optimum Conditions for the Willgerodt-Kindler Reaction. 1: Reaction of Substituted Acetophenones. Prediction of Optimum Conditions for New Substrates by Multivariate Correlation.
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Creatine kinase in serum: 1. Determination of optimum reaction conditions.
To establish optimum conditions for creatine kinase (EC 2.7.3.2) activity measurement with the creatine phosphate in equilibrium creatine reaction, we re-examined all kinetics factors relevant to an optimal and standardized enzyme assay at 30 and 25 degrees C. We determined the pH optimum in vaious buffers, considering the effect of the type and concentration of the buffer, as well as the influ...
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Creatine kinase: re-examination of optimum reaction conditions.
I have re-examined optimum reaction conditions for measurement of creatine kinase (EC 2.7.3.2). The optimum pH is 6.45, and 2,2-bis(hydroxymethyl)-2,2',2''-nitrotriethanol acetate, 200 mmol/liter, is the buffer of choice. Thioglycerol, 20 mmol/liter, is superior for both in-assay reactivation and for storage stability of sera. Fluoride, 25 mmol/liter, a broad inactivator of adenylate kinase (EC...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1986
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.40b-0534